Urine Collection (24 Hour)

Your doctor may order a ‘24’ hour urine to study your body’s function over an exact 24 hour period.  It is very important to follow these instructions carefully to obtain a specimen suitable for testing.  The 24 hour jug should be provided to you by your physician’s office or can be picked up at any AdventHealth outpatient laboratory or patient service center.

Patient Preparation

  1. Avoid alcoholic beverages and vitamins for at least 24 hours before starting to collect your urine and during the collection period.
  2. Do not change your dietary habits before the test unless your physician instructs you to.
  3. Do not discontinue medications unless instructed to do so by your physician.
  4. Do not urinate directly into the 24-hour container. You should urinate in a sterile container and carefully pour it into the 24-hour container, without losing any specimen.
  5. Keep the 24-hour urine container refrigerated (or in a cooler) between each urine collection.

Urine Collection

  1. First thing in the morning, start the 24 hour urine test by urinating directly into the toilet. Do not save this urine. After you urinate, write the date and time (along with your name and date of birth) on your storage container. Even though this urine is not saved, the test time begins now.
  2. From this point on, collect ALL urine during the next 24 hours for both day and night (Be sure to wash your hands before and after you urinate).
  3. Urine passed during bowel movements must be saved too.  Be careful to include only urine in the sterile container.
  4. Try to urinate at about the same time the next morning.  After pouring this urine in the 24-hour container, be sure to indicate the end time on the container. This will be your last collection.
  5. Take the 24-hour specimen to the laboratory as soon as possible after finishing. Try to keep the urine cool during transport by using ice pack.